Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0; Protein content: > 95%; Texas Red: A max. 596 nm; E max. 620 nm. Fluorochrome / protein Absorbance ratio: A596 nm/ A280 nm.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application
Recommended Dilution
ICC/IF
Assay dependent
FACS
Assay dependent
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
10mM Phosphate, 150mM NaCl, 1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. Protect from light.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Conjugation
Texas Red (TxRd)
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Background
Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0; Purity: Streptavidin used is 99% pure by HPLC; Eletrophoretically homogenous, single band, Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Texas Red: A max. 596 nm; E max. 620 nm. Fluorochrome / protein Absorbance ratio: A596 nm/ A280 nm.